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. 2017 Jun 20;65(6):1014–1020. doi: 10.1136/jim-2017-000426

Figure 1.

Figure 1

Effect of different chemicals on cell apoptosis in MC3T3-E1 cells. (A) MC3T3-E1 cells were treated vehicle control, 5-fluorouracil (5-FU) (20 µg/mL, 36 hours), rapamycin (500 ng/mL, 6 hours), 3-methyl adenine (3-MA) (10 nM, 12 hours), tumor necrosis factor-α (TNF-α) (20 ng/mL, 48 hours), rapamycin+TNF-α (rapamycin, 500 ng/mL, 6 hours; TNF-α, 20 ng/mL, 48 hours) or 3-MA+TNF-α (3-MA, 10 nM, 12 hours; TNF-α, 20 ng/mL, 48 hours). Cells were stained with Annexin-V-FITC and propidium iodide (PI). The apoptotic rate was determined by flow cytometry. (B) Quantitative data of Annexin-V-FITC/PI staining. Data were presented as mean±SD in triplicate. *p<0.05 versus corresponding control.