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. 2017 Jul 27;8:15990. doi: 10.1038/ncomms15990

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Model of a Drosophila larva; hemocytes in red, neurons in green. Boxed area marks a HP. (b) Close-up of a lower part of a HP illustrating intricate neuronal extensions in areas of hemocytes; neurons (Anti-HRP, green), hemocytes (HmlΔ-DsRed, red), lateral patch area. Middle and lower panel show single channels. (c) Actβ expressing larval PNS neurons marked by reporter Actβ-GAL4; UAS-mCD8GFP (green), Actβ positive neurons colocalize with hemocytes marked by HmlΔ-DsRed (red). Fillet prep containing all tissue layers. (d) Actβ expression pattern in larval PNS neurons, Actβ-GAL4/+; UAS-r-stinger/+ (green), pan-neuronal anti-elav (red). Fillet prep containing all tissue layers. (e) Model of Actβ expression (green) in most multidendritic neurons (diamonds), chordotonal organs (triangles), and some external sensory neurons (circles). Scale bars, b, 50 μm; c,d, 100 μm.