Figure 2.

Brains coexpressing Ras^V12 and pico display extracellular matrix degradation and ectopic expression of Mmp1. (a) Optic lobes from larvae overexpressing pico, Ras^V12 or Ras^V12, pico under the control of eyFLP, Act>GAL4, stained with anti-Laminin antibody, which labels the surface of the optic lobes. Laminin staining was found to be severely interrupted in brains coexpressing Ras^V12 and pico but not from brains expressing pico or Ras^V12 alone. (b) Distribution of the metalloproteinase Mmp1. Little or no Mmp1 staining was observed in animals expressing Ras^V12 or pico alone. In contrast, animals co-overexpressing Ras^V12 and pico had elevated Mmp1 around the edges of the optic lobes and at sites of invasion into the VNC (arrow).