Table 1. Average size of DNA fragments at various stages during the protocol.
The table shows the modal fragment size post-shear and post-PCR, and mean Nanopore read length (‘pass’, and ‘fail’ read quality) with standard deviations (sd), of the samples used in this study.
| Samples | Average (modal) size of DNA fragments | Average (mean) read length | ||
|---|---|---|---|---|
| Post-shear (nt) | Post-PCR (nt) | ‘Pass’ reads [nt (sd)] | ‘Fail’ reads [nt (sd)] | |
| Influenza virus A non-enriched* | 160, 320, 500, 670, 900, 1250, 3000+* | 99–4000 | 1598 (1191) | 805 (946) |
| Influenza virus A enriched* | 160, 320, 500, 670, 900, 1250, 3000+* | 370–4000 | 773 (683) | 533 (733) |
| HCMV non-enriched | 12 500† | –‡ | 3176 (2291) | 487 (1203) |
| HCMV enriched | 12 500 | 1587, 5640 | 1528 (975) | 1083 (1099) |
| M. tb H37Rv | 13 800 | 2000–7000 | 2402 (1865) | 757 (1855) |
| M. tb strain C | 15 000 | 1500 | 759 (355) | 596 (713) |
*Influenza virus A samples were not sheared.
†HCMV fragment size after shearing and PreCR.
‡The non-enriched HCMV sample was not PCR-amplified as enough material was available to proceed directly to sequencing.