Figure 6.

Caveolin-1 is indispensable for the increased metastasis resulting from NDRG1 depletion in vivo. (a) Mice were imaged at 3 weeks after injection using Xenogen. Four representative mice were imaged and the color scale depicting the photon fluxes emitted from the tumor cells. (b) Representative images taken at 8 weeks after injection. (c) The representative images show macroscopic observations of metastases in lung at the time of killing. (d) The time of first tumor appearance of each experimental group. Results are collected and analyzed from 10 nude mice per group and the histograms represent mean±s.d. ***P<0.001, relative to the respective control cells. (e) Metastases in hematoxylin and eosin (H&E)-stained lung sections; magnification: × 40. (f) Metastases were counted on five lobes of the lung in all animal groups. The results are collected and analyzed from 10 nude mice per group and the histograms represent mean±s.d. number of metastatic nodules. ***P<0.001, relative to the respective control cells. (g) The representative IHC staining images of NDRG1 and caveolin-1 in lung metastatic tissues; magnification: × 40.