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. 2017 Mar 27;36(30):4323–4335. doi: 10.1038/onc.2017.74

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Immunoprecipitation for NDRG1 and caveolin-1 in three CRC cell lines. Immunoprecipitation of 3 × Flag-tagged NDGR1 protein was performed using M2 beads. Caveolin-1 immunoprecipitation was performed by using protein G plus agarose beads. Caveolin-1 and NDRG1 expressions were detected by immunoblots. Caveolin-1 was detected alone in the precipitates derived from the cells transfected with 3 × Flag-NDRG1, and was not detected in the negative control group. Reciprocally, after immunoprecipitation with anti-caveolin-1 antibodies, NDRG1 was also detected in the co-immunoprecipitation (co-IP) complex. These results indicated that there was a direct interaction between caveolin-1 and NDRG1 in vitro.