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. 2017 Jun 13;7(6):e1152. doi: 10.1038/tp.2017.111

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Generation of brain-specific Chd5^−/− mice. (a) Chd5 gene-targeting strategy. See Materials and methods for primer (P1–3) information. (b) Breeding strategy for brain-specific Chd5^−/− mice (box, schematic of tomR-GFP reporter gene expression). PCR genotyping of genomic DNA from (c) tail biopsy for (top) Chd5-targeted allele and (bottom) nestin-Cre transgene allele and (d) whole-brain extract for Chd5 null allele. (e) Representative Chd5^−/− brain expressing TomR (red) in non-neural and GFP (green) in neural tissue. Inset represents magnified region. Scale bar, 100 μM. (f) Western blot of whole-brain extracts indicating the near absence of protein in Chd5^−/− mice compared with Chd5^+/+ mice (n=6/group). **P=0.0002. Chd5, chromodomain helicase DNA-binding 5; GFP, green fluorescent protein; PCR, polymerase chain reaction.