Skip to main content
. 2017 Mar 2;11(8):895–912. doi: 10.1002/1878-0261.12035

Figure 5.

Figure 5

(A) Exemplificative annotated nanoLC‐ESI‐LTQ‐Orbitrap‐CID‐MS/MS spectra for a MUC16 glycopeptide substituted with a HexNAc residue evidencing the specific glycosite; (B) colocalization of MUC16 and STn in bladder tumours by immunohistochemistry; (C) expression of MUC16 STn glycoforms in bladder tumours based on PLA analysis. This work identified for the first time MUC16 in bladder tumours and its association with abnormal glycoforms such as the STn antigen. The mass spectrum shows a MUC16 glycopeptide substituted with a HexNAc residue, strongly suggesting the presence of STn. The colocalization of MUC16 and STn (B) in bladder tumours also reinforces this hypothesis. Finally, the red dots on the PLA image (C) in areas of colocalization result from the simultaneous detection of both antigens, reinforcing this evidence.