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. 2017 May 30;11(8):981–995. doi: 10.1002/1878-0261.12066

Figure 2.

Figure 2

Pharmacologic inhibition of AURKA with MLN8237 downregulates phosphorylation of eIF4E and protein expression of downstream effectors in CDDP‐resistant cells. AGS CDDPR Pool 1, AGS CDDPR Pool 2, AGS CDDPR Clone 1, and AGS CDDPR Clone 2 cells were treated with MLN8237 (0.5 μm), and cell lysates were subjected to western blot analysis of the indicated proteins. The data showed that pharmacologic inhibition of AURKA with MLN8237, as indicated by decreased protein levels of p‐AURKA (T288), reduced the protein levels of p‐eIF4E (S209) and its protein translation targets, c‐MYC and HDM2 in CDDP‐resistant cells. Gel loading was normalized for equal β‐actin.