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. 2001 Jul 31;98(16):9086–9091. doi: 10.1073/pnas.161290898

Figure 6.

Figure 6

Fura-2 measurements of [Ca2+]i in cocultured cells. (A) Pseudocolor images of HEK293 cells cotransfected with GFP and P2Y2 (HEK-P2Y2-GFP, ATP “sensors”) in coculture with NCI H292 cells (ATP “releasers”) at three different time points. Representative HEK-P2Y2-GFP cell identified retrospectively by its GFP label is circled and shows an increase in intracellular Ca2+ 24 s after the addition of α-ASGM1 (1:40), which recovers by 52 s (typical of 28 cells, n = 5 experiments). (B) Fura-2 ratios showing increased [Ca2+]i 20 s after the addition of α-ASGM1 in ATP “sensing,” GFP-labeled HEK293 cells, followed by stimulation with 100 μM ATP as a control. Cells were imaged in the same high-power (×20) field while in coculture with ATP “releasers,” NCI H292 cells.