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. 2017 Jun 5;11(8):1078–1098. doi: 10.1002/1878-0261.12076

Figure 5.

Figure 5

Combined TMZ and dovitinib treatment increases cell death. GB cells were treated with 100 μm TMZ alone, 2 μm and 5 μm Dov alone or in combination with 100 μm TMZ and the percentage of apoptotic cells (sub‐G1 population) was quantified by PI flow cytometry after 48 h in GB‐10 (A), U251 (B), and U251/HMGA2‐overexpressing (C) cells. The % apoptotic cell fraction gated in M2 (sub‐G1 subpopulation) is indicated for each cell line and treatment. HMGA2 overexpression mitigated the apoptotic response to Dov alone and to the combined Dov/TMZ treatment, and this effect was pronounced at 5 μm Dov (C). Representative FACS results of three independent experiments are shown.