Figure 8.

Alternating dovitinib/TMZ treatments reduce cell survival upon recovery. (A) Schematic representation of the alternating Dov (5 μm) and TMZ (100 μm) treatment plan, 3 days each and 1 day of 5 μm Dov, for U251 and GB‐10 followed by a nine‐day cell recovery phase in normal medium without drugs. Survival of GB cells was determined by their ability to form colonies as quantified at the end of the recovery time. (B) Representative phase‐contrast images are shown. (C) Alternating treatments with Dov and TMZ every 3 days for a total of 15 days followed by a recovery phase for 9 days (I) significantly reduced the number of colonies by >75% when compared to alternating treatment with TMZ alone (II), as shown for U251. Graphs show SEM from three independent experiments; ***P < 0.001.