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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Biomaterials. 2017 Jun 9;140:26–36. doi: 10.1016/j.biomaterials.2017.06.012

Figure 5.

Figure 5

US was used to control the release of bioactive, bFGF from an ARS (A) The percent of bFGF released as a function of acoustic pressure where “Daily” indicates US exposure on days 1–7. A delayed release experiment was also performed (i.e., “Delayed”) where US was applied on days 4–7. (B) The bioactivity of the released growth factor was determined by incubating NR-6-R fibroblasts with releasate. Cell proliferation was measured after 44 hours. Releasates obtained at 0 or 2 MPa did not contain enough bFGF to induce cell proliferation. All data is represented as mean ± standard error of the mean for n = 5 ARSs. For (A), statistically significant differences (p < 0.05) are denoted as follows. α: 8 MPa (daily) vs. –US; β: 4 MPa (daily) vs. –US; χ: 8 MPa (daily) vs. 4 MPa (daily); ε: 8 MPa (daily) vs. 8 MPa (delayed); η: 4 MPa (daily) vs. 8 MPa (delayed); δ: 8 MPa (delayed) vs. –US. For (B), statistically significant differences (p < 0.05) are denoted as follows. α: vs. fibrin+bFGF on day 2, β: vs. fibrin+bFGF on day 6.