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. Author manuscript; available in PMC: 2018 Jul 18.
Published in final edited form as: Circulation. 2017 Apr 25;136(3):281–296. doi: 10.1161/CIRCULATIONAHA.116.024873

Figure 2. Strain-specific coding variants of BAG3 differentially promote limb muscle hypertrophy and capillary density in non-ischemic muscle.

Figure 2

A–B. Intramuscular injection into BALB/c mice of adeno-associated viruses encoding BAG3Met81 or BAG3Ile81 (N=4 mice/virus) resulted in similar upregulation of Bag3 mRNA on day 3 post-injection (A) and FLAG-tagged BAG3 protein on day 7 post-injection (B) compared to mice injected with AAV-GFP. * P<0.05 vs. GFP. IP, immunoprecipitation; WB, western blot. C. Representative dystrophin staining (pseudocolored green; scale bar=100 μm) of TA muscle 14 days post-injection. D. Quantification of myofiber CSA on days 7 and 14 post-injection (N=4 mice/virus). ‡ P<0.05 vs. GFP or BAG3Met81. E. Representative images of CD31 and smooth muscle actin (SMA) staining 7 days post-injection (scale bar=100 μm). F. Quantification of CD31+ vessel density in TA muscle. *, P<0.05 vs. GFP (N=4 mice/virus). G–H. Limb perfusion was analyzed by LDPI on day 7 post-injection (N=10 mice/virus) (G) and quantified (H) as a percentage of perfusion in the non-injected limb.