Figure 1.

Cxcl14 is expressed in muscle cells. (a) C2C12 cell media over the course of differentiation (0, 24, 48, 72 h) were subjected to ELISA assay to determine secreted Cxcl14 levels (n=5, samples assayed in duplicates). (b) TA muscles were injured by BaCl₂ injection and isolated on days 3, 5, 7 and 14 after injury (AI). Upon cryosection, H&E staining or immunofluorescence staining for Cxcl14 (green) together with DAPI (blue) was performed (n=3). Scale bars: 50 μm. (c) The procedure described in (b) was repeated. Muscle sections isolated on day 3 AI were immunofluorescently probed for MyoD (red), Cxcl14 (green) and DAPI (blue) (n=6). Scale bar: 15 μm. Paired two-tailed t-test was performed for the data in (a). The data denoted by different letters (a–c) are significantly different from each other (P<0.05). All error bars represent s.d. of independent replicates.