Figure 4.

ERK1/2 mediates Cxcl14’s anti-myogenic effect. (a) C2C12 cells were infected with shRNA lentiviruses and selected, followed by cell lysis and western analysis (n=4). (b) Cells were treated as in (a), differentiated for 24 h, then stimulated with 10 ng/ml rCxcl14 for the indicated amount of time, followed by cell lysis and western analysis (n=3). (c) C2C12 cells were infected with shRNA lentiviruses as indicated, then grown in the presence or absence of 25 ng/ml rCxcl14 and U0126 for 24 h. Cells were then differentiated for 72 h and subsequently stained for MHC and with DAPI, followed by quantification of the fusion index (n=3). Scale bar: 50 μm. Paired two-tailed t-test was performed for the data in (c). The data denoted by different letters (a–c) are significantly different from each other (P<0.05). All error bars represent s.d. of independent replicates.