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. 2017 Jun 23;9(7):650. doi: 10.3390/nu9070650

Figure 1.

Figure 1

Effect of quercetin on microglia activation treated with adipose tissue conditioned medium (ATCM) or free fatty acid (FFA). Microglia (BV2) were treated with ATCM in the presence or absence of quercetin (5, 10 μM) for 24 h. (A) The transcription levels of iNOS, MCP-1, and IL-10 were measured by RT-PCR analysis; (B) The levels of MCP-1 in the conditioned-medium were measured by ELISA; (C,D) Microglia were treated with FFA (palmitic acid) (300 μM) for 6 h. The transcription levels of inflammatory cytokines (MCP-1, TNFα, IL-1β) were measured by RT-PCR analysis. Data are the means ± SEM of three independent experiments performed in triplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with control.