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. 2017 Jun 24;9(7):654. doi: 10.3390/nu9070654

Figure 1.

Figure 1

Effect of a fish oil diet on acetylcholine-dependent vasorelaxation in thoracic aortas. Protein expression of soluble epoxide hydrolase (sEH) in rat aortic strips, rat aorta endothelial cells (RAECs), and rat vascular smooth muscle cells. (A) Concentration-vasodilatory response curves induced by acetylcholine in aortic strips obtained from Sprague-Dawley (SD) rats, fed a control diet (black circles, black line) or fish oil diet (open circles, blue line) for 3 weeks. Results are expressed as the mean ± standard error of the mean (SEM) of six strips. (B) The protein expression of sEH, platelet endothelial cell adhesion molecule-1 (PECAM-1) (endothelial marker) and β-actin in aortic strips from control or fish oil diet-fed rats. n = 3. (C) The protein expression of cytochrome P450 2J2 (CYP2J2) and sEH in RAECs and rat vascular smooth muscle cells was examined by western blotting. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with control. IB: immunoblotting.