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. 2017 Jun 24;9(7):654. doi: 10.3390/nu9070654

Figure 2.

Figure 2

Effect of docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) on sEH protein expression and epoxyeicosatrienoic acid/dihydroxyeicosatrienoic acid (EET/DHET) ratio. (A, B) The sEH protein expression in RAECs incubated with 100 μM DHA or EPA for 24 and 48 h was evaluated by western blotting and normalized against β-actin. (C, D) RAECs were incubated with 50 or 100 μM DHA or EPA for 48 h, and the sEH protein expression was examined. (E, F) RAECs were incubated with 100 µM DHA or EPA for 48 h, and the intracellular EET/DHET ratio was measured. 11, 12-EET, 14, 15-EET, 11, 12-DHET and 14, and 15-DHET were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Each value represents the mean ± SEM. (A) n = 25 (bovine serum albumin (BSA)), 3 (100 µM DHA, 24 h), 22 (100 µM DHA, 48 h); (B) n = 13 (BSA), 3 (100 µM EPA, 24 h), 10 (100 µM EPA, 48 h); (C) n = 32 (BSA), 10 (50 µM DHA, 48 h), 22 (100 µM DHA, 48 h); (D) n = 13 (BSA), 3 (50 µM EPA, 48 h), 10 (100 µM EPA, 48 h); (E, F) n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with control. BSA: Fatty acid-free bovine serum albumin.