Skip to main content
. 2017 Jun 24;9(7):654. doi: 10.3390/nu9070654

Figure 3.

Figure 3

Effect of DHA and EPA on intracellular 4-hydroxy hexenal (4-HHE) levels. Effect of 4-HHE on sEH expression. (A, B) The concentration of intracellular 4-HHE in RAECs treated with 100 µM DHA or EPA was measured by LC-MS/MS analysis. (C, D) RAECs were incubated with 15 or 30 µM 4-HHE for 24 and 48 h, and the protein expression of sEH and β-actin was examined by western blotting. (E–G) RAECs were incubated with 100 µM DHA or EPA, or 15 µM 4-HHE for 24 and 48 h. The relative mRNA level of epoxide hydrolase 2 (EPHX2) was quantified using real-time quantitative PCR (RT-qPCR). Each value represents the mean ± SEM. (A, B) n = 3; (C) n = 19 (control), 3 (15 µM 4-HHE, 24 h), 16 (15 µM 4-HHE, 48 h); (D) n = 25 (control), 16 (15 µM 4-HHE, 48 h), 9 (30 µM 4-HHE, 48 h); (E–G) n = 6 (BSA), n = 3 (100 µM DHA or EPA, 15 µM 4-HHE, 24 or 48 h). * p < 0.05, ** p < 0.01, *** p < 0.001, compared with control.