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. 2017 Jun 24;9(7):654. doi: 10.3390/nu9070654

Figure 6.

Figure 6

Effect of the p38 kinase inhibitor (SB203580) on DHA, EPA, or 4-HHE-suppression of sEH protein expression. (A) RAECs were incubated with DHA (100 µM), EPA (100 µM), or 4-HHE (15 µM) for 48 h. p-p38 and p38 levels were determined by western blotting. n = 3. (B–D) RAECs were treated with the p38 kinase inhibitor SB203580 (10 µM) for 30 min before control, DHA (100 µM), EPA (100 µM), or 4-HHE (15 µM) incubation. sEH and β-actin protein levels were determined by western blotting. Each value represents the mean ± SEM. n = 6–7. (B–D) One-way ANOVA followed by Tukey’s HSD post hoc analysis. * p < 0.05, ** p < 0.01. NS, no significant difference.