Figure 5.
Effects of EGCG on activation of mTOR and AMPK in adipocytes. (A,B) Immunoblot analysis of phosphorylation of mTOR and AMPK in adipocytes differentiated from C3H10T1/2 treated with starvation medium, 8Br-cAMP (1 mM) and EGCG (10 μM) for 24 h. Mitochondrial membrane potential (C), and intracellular ATP (D) in adipocytes differentiated from C3H10T1/2 treated with 8Br-cAMP (1 mM), FCCP (0.5 μM), EGCG (10 or 20 μM) or quercetin (10 or 20 μM) for 2 h. p values were calculated using the two-tailed unpaired t-test (n = 3, means ± SE; * p < 0.05, ** p < 0.01, *** p < 0.001).