Figure 6.
Effects of knockdown of RAB7 on EGCG induced reduction in lipid content in adipocytes. (A) Immunoblot analysis of RAB7 in adipocytes differentiated from C3H10T1/2 treated with siRNA or scrambled sequence controls. Two-way ANOVA revealed significant main effects of siRNA knockdown (p < 0.0001) and EGCG treatment (p = 0.0122) in RAB7 expression and significant interaction of siRNA knockdown and EGCG treatment (p = 0.0206). Significant differences between control and siRNA were determined by post-hoc pairwise comparison with Bonferroni correction (mean ± SEM; n = 4 per condition, *** p < 0.001) (B) Representative images of adipocytes differentiated from C3H10T1/2 treated with vehicle or EGCG (10 μM) for 24 h. (n = 4, means ± SE; *** p < 0.001).