Table 1.
Composition of experimental diets 1.
| Component (g/kg Diet) | Control Diet | MC Diet | HGPC Diet |
|---|---|---|---|
| Casein | 270 | 270 | 270 |
| Starch | 240 | 240 | 240 |
| Sucrose | 126 | 126 | 126 |
| Vitamin mix (AIN-93-Vx) 2 | 19 | 19 | 19 |
| Mineral mix 3 | 50 | 50 | 50 |
| Calcium phosphate dibasic | 3.4 | 3.4 | 3.4 |
| Inositol | 6.3 | 6.3 | 6.3 |
| Cellulose | 80.0 | 80.0 | 80.0 |
| l-cysteine | 1.8 | 1.8 | 1.8 |
| Fat mixture | |||
| Canola oil | 40 | 31 | 39 |
| Olive oil | 13 | 13.8 | 8.5 |
| Vegetable oil | 14 | 14 | 14.3 |
| Corn oil | 73 | 65 | 71.9 |
| Sunflower oil | 2 | 5 | 2.8 |
| Flax seed oil | 2 | 2 | 2 |
| Hydrogenated canola oil | 56 | 57.5 | 56 |
| DHAsco | 1.5 | 1.5 | 1.5 |
| ARAsco | 1.5 | 1.5 | 1.5 |
| Choline mixture (providing 1 g of total choline/kg diet) | |||
| Soy lecithin (PC) | 0 | 13.1 | 3.2 |
| Choline bitartrate | 2.1 | 0.6 | 0.3 |
| Glycerophosphocholine | 0.0 | 0.6 | 1.9 |
GPC, glycerophosphocholine; LysoPC, lysophosphatidylcholine; PC, phosphatidylcholine. 1 All ingredients were purchased from Harlan Teklad (Indianapolis, IN, USA), with the exception of the dietary oils that were all purchased from Safeway (Edmonton, AB, Canada). The hydrogenated canola oil was donated by Richardson Oilseed Limited (Lethbridge, AB, Canada) and ARAsco and DHAsco were donated by DSM (Nutritional Products, Columbia, MD, USA); 2 AIN-93-VX Vitamin mix [7]; 3 Bernhart–Tomarelli salt mixture [19].