Table 4.
Splenocyte phenotypes of lactating dams fed Control (100% FC), Mixed Choline (MC; 50% PC, 25% FC, 25% GPC) or High GPC (HGPC; 75% GPC, 12.5% PC, 12.5% FC) diets.
| Cell Phenotype | Control (n = 6) | MC (n = 6) | HGPC (n = 6) | p Value |
|---|---|---|---|---|
| % of total lymphocytes | ||||
| Total CD3+ (T cell) | 44.6 ± 1.8 | 40.9 ± 1.8 | 36.9 ± 3.5 | 0.167 |
| % of CD3+ cells | ||||
| CD4+ | 59.3 ± 1.7 a | 57.5 ± 4.6 a | 51.1 ± 2.7 b | 0.011 |
| CD8+ | 34.8 ± 0.9 | 32.1 ± 1.5 | 36.1 ± 1.7 | 0.164 |
| % of CD4+ T cells | ||||
| CD27+ | 73.7 ± 9.5 | 81.8 ± 2.7 | 86.3 ± 2.8 | 0.342 |
| CD28+ | 84.2 ± 6.1 b | 98.9 ± 0.6 a | 94.7 ± 1.7 ab | 0.035 |
| CD71+ | 6.9 ± 1.1 b | 11.1 ± 1.0 a | 13.4 ± 1.2 a | 0.041 |
| CD127+ | 2.9 ± 0.5 | 2.1 ± 0.2 | 2.6 ± 0.6 | 0.558 |
| % of CD8+ T cells | ||||
| CD27+ | 79.3 ± 8.1 b | 98.2 ± 1.3 a | 99.7 ± 0.2 a | 0.013 |
| CD28+ | 71.7 ± 6.3 | 74.1 ± 1.4 | 69.1 ± 2.4 | 0.683 |
| CD71+ | 7.9 ± 0.8 b | 13.2 ± 0.9 ab | 16.4 ± 3.2 a | 0.023 |
| CD127+ | 3.9 ± 0.9 b | 5.1 ± 0.3 a | 8.2 ± 1.2 a | 0.011 |
| % of total lymphocytes | ||||
| CD68+CD284+ | 8.3 ± 0.3 ab | 7.9 ± 0.2 b | 9.0 ± 0.3 a | 0.045 |
| OX62+OX6+ (Dendritic cell) | 4.4 ± 1.1 b | 8.6 ± 0.5 a | 9.2 ± 0.6 a | 0.001 |
| Total CD45RA+ (B cells) | 30.7 ± 1.0 b | 40.4 ± 1.3 a | 39.1 ± 4.7 a | 0.034 |
| CD80+CD45RA+ | 4.8 ± 0.5 a | 2.9 ± 0.2 b | 3.7 ± 0.2 b | 0.004 |
| IgA+ | 12.1 ± 0.6 | 12.1 ± 0.3 | 13.6 ± 0.6 | 0.105 |
| IgG+ | 10.4 ± 1.1 a | 5.4 ± 0.8 b | 3.2 ± 0.2 b | 0.0003 |
| IgM+ | 49.6 ± 1.5 b | 55.2 ± 0.8 a | 53.1 ± 1.9 ab | 0.045 |
FC, free choline; GPC, glycerophosphocholine; LysoPC, lysophosphatidylcholine; PC, phosphatidylcholine. Values are presented as mean ± SEM. p value of the main effect of diet analyzed by one-way ANOVA. Multiple comparisons between diet groups have been performed with Duncan adjustment. Means within a row that do not share a common superscript letters a, b are significantly different (p < 0.05). The following phenotypes not shown in table were not significantly different among groups (mean ± SEM, % of total cells, n = 18): CD4+CD25+, 7.0 ± 0.5; CD8+CD25+, 4.7 ± 0.5; CD4+CD152+, 2.7 ± 1.1; CD8+CD152+, 8.4 ± 1.2; CD3-CD161+, 2.5 ± 0.3; CD3+CD161+, 0.8 ± 0.2; OX6+OX12+, 26.3 ± 1.6; OX6+CD80+, 4.9 ± 0.6; OX12+CD80+, 4.4 ± 0.5; CD27+CD45RA+, 2.8 ± 0.7.