Table 5.
Mesenteric lymphocyte phenotypes of lactating dams fed Control (100% FC), Mixed Choline (MC; 50% PC, 25% FC, 25% GPC) or High GPC (HGPC; 75% GPC, 12.5% PC, 12.5% FC) diets.
| Cell Phenotype | Control (n = 6) | MC (n = 6) | HGPC (n = 6) | p Value |
|---|---|---|---|---|
| % of total lymphocytes | ||||
| Total CD3+ (T cell) | 57.0 ± 1.6 | 58.5 ± 1.1 | 60.3 ± 1.5 | 0.278 |
| % of CD3+ cells | ||||
| CD4+ | 69.3 ± 0.6 | 70.1 ± 1.4 | 66.0 ± 1.4 | 0.061 |
| CD8+ | 26.0 ± 1.6 | 26.5 ± 3.8 | 22.4 ± 3.7 | 0.628 |
| % of CD4+ T cells | ||||
| CD71+ | 6.2 ± 0.9 | 5.5 ± 0.8 | 6.8 ± 0.6 | 0.484 |
| CD127+ | 0.73 ± 0.2 | 1.5 ± 0.3 | 2.7 ± 1.2 | 0.215 |
| CD152+ (CTLA-4+) | 1.8 ± 1.1 | 0.8 ± 0.1 | 2.4 ± 0.9 | 0.433 |
| % of CD8+ T cells | ||||
| CD71+ | 7.0 ± 0.7 | 7.9 ± 1.3 | 5.7 ± 0.5 | 0.258 |
| CD127+ | 1.0 ± 0.2 | 1.1 ± 0.1 | 1.9 ± 0.5 | 0.104 |
| CD152+ (CTLA-4+) | 5.7 ± 0.5 b | 5.4 ± 0.4 ab | 7.9 ± 0.6 a | 0.005 |
| % of total lymphocytes | ||||
| Total CD45RA+ (B cells) | 26.8 ± 1.3 | 36.1 ± 1.3 | 33.7 ± 0.1 | 0.554 |
| IgA+ | 4.3 ± 0.6 a | 1.6 ± 0.1 b | 2.3 ± 0.3b | 0.001 |
| IgG+ | 6.0 ± 0.9 a | 3.4 ± 0.2 b | 5.3 ± 0.5 ab | 0.024 |
| IgM+ | 38.7 ± 1.9 | 32.1 ± 2.9 | 37.4 ± 1.6 | 0.335 |
FC, free choline; GPC, glycerophosphocholine; LysoPC, lysophosphatidylcholine; PC, phosphatidylcholine. Values are presented as mean ± SEM. p value of the main effect of diet analyzed by one-way ANOVA. Multiple comparisons between diet groups have been performed with Duncan adjustment. Means within a row that do not share a common superscript letters a, b are significantly different (p < 0.05). The following phenotypes not shown in table were not significantly different among groups (mean ± SEM, % of total cells, n = 18); CD4+CD25+, 7.8 ± 0.9; CD4+CD27+, 62.3 ± 1.5; CD4+CD28+, 70.1 ± 3.6; CD8+CD25+, 4.0 ± 0.4; CD8+CD27+, 66.3 ± 2.5; CD8+28+, 65.1 ± 4.0.