Table 1.

Bacterial strains, assay conditions for the minimum inhibitory concentration (MIC), and MIC results of equol to the intestinal species and strains under study.

Bacterial Strains		MIC Assay Conditions			MIC Results
Bacterial Group/Species	Strain Code	Medium	Temperature	Atmosphere	Equol (μg mL−1)
Lactic acid bacteria
Lactococcus (Lc.) lactis subsp. cremoris	LMG 6987T	IST a	32 °C	Aerobiosis	256
Lc. lactis subsp. lactis	LMG 6890T	IST	32 °C	Aerobiosis	128
Streptococcus termophilus	LMG 6896T	IST-Lac b	37 °C	Anaerobiosis	256
Lactobacillus (Lb.) brevis	LMG 6906T	LSM c	32 °C	Aerobiosis	256
Lb. casei	LMG 6904T	LSM	32 °C	Aerobiosis	1024
Lb. fermentum	LMG 6902T	LSM	37 °C	Aerobiosis	1024
Lb. paracasei subsp. paracasei	LMG 13087T	LSM	32 °C	Aerobiosis	1024
Lb. pentosus	LMG 10755T	LSM	32 °C	Aerobiosis	1024
Lb. plantarum	LMG 6907T	LSM	32 °C	Aerobiosis	1024
Lb. reuteri	LMG 9213T	LSM	37 °C	Aerobiosis	512
Lb. rhamnosus	LMG 6400T	LSM	37 °C	Aerobiosis	512
Lb. sakei subsp. sakei	LMG 9468T	LSM	32 °C	Aerobiosis	256
Lb. acidophilus	LMG 9433T	LSM-Cys d	37 °C	Anaerobiosis	512
Lb. delbrueckii subsp. bulgaricus	LMG 6901T	LSM-Cys	37 °C	Anaerobiosis	64
Lb. delbrueckii subsp. delbrueckii	LMG 6412T	LSM-Cys	37 °C	Anaerobiosis	256
Lb. delbrueckii subsp. lactis	LMG 7942T	LSM-Cys	37 °C	Anaerobiosis	128
Lb. gasseri	LMG 9203T	LSM-Cys	37 °C	Anaerobiosis	128
Lb. helveticus	LMG 6413T	LSM-Cys	37 °C	Anaerobiosis	1024
Lb. johnsonii	LMG 9436T	LSM-Cys	37 °C	Anaerobiosis	512
Bifidobacteria
Bifidobacterium (B.) adolescentis	LMG10502T	LSM-Cys	37 °C	Anaerobiosis	256
B. animalis subsp. animalis	LMG 10508T	LSM-Cys	37 °C	Anaerobiosis	16
B. animalis subsp. lactis	E43	LSM-Cys	37 °C	Anaerobiosis	128
B. breve	LMG 13208T	LSM-Cys	37 °C	Anaerobiosis	256
B. longum subsp. longum	LMG 13197T	LSM-Cys	37 °C	Anaerobiosis	256
B. pseudolongum subsp. pseudolongum	LMG 11571T	LSM-Cys	37 °C	Anaerobiosis	128
B. termophilum	LMG 21813T	LSM-Cys	37 °C	Anaerobiosis	256
Other intestinal bacteria
Bacteroides (Bact.) fragilis	DSM 2151T	M1 e	37 °C	Anaerobiosis	64
Bact. thetaiotaomicron	DSM 2079T	M1	37 °C	Anaerobiosis	64
Blautia coccoides	DSM 935T	M1	37 °C	Anaerobiosis	256
Faecalibacterium prausnitzii	DSM 17677	M1	37 °C	Anaerobiosis	256
Ruminococcus obeum	DSM 25238T	M1	37 °C	Anaerobiosis	256
Slackia (Sl.) equolifaciens	DSM 24851T	M1	37 °C	Anaerobiosis	64
Sl. isoflavoniconvertens	DSM 22006T	M1	37 °C	Anaerobiosis	1024
Escherichia coli	E-73	IST	37 °C	Aerobiosis	2048
Klebsiella pneumoniae	K-78	IST	37 °C	Aerobiosis	2048
Pseudomonas aeruginosa	PS-25	IST	37 °C	Aerobiosis	1024
Serratia marcescens	S-54	IST	37 °C	Aerobiosis	512

^a IST, IsoSensitest (Oxoid); ^b IST-Lac (IST + 1% lactose); ^c LSM, Lactic acid bacterium susceptibility test medium (90% IST + 10% de Man, Rogosa and Sharpe (MRS)); ^d LSM-Cys (LSM + 0.03% cysteine); ^e M1 (90% IST + 10% Gifu Anaerobic Medium (GAM) + 0.25% cysteine). MICs were assayed in duplicate or triplicate; when discrepancies were found, the mode was reported.