Table 2.
Effect of the diets on bone parameter values by µCT and 3-point bending assay.
| Bone Parameters | Control 1,3 Diet (CD) (n = 8) (Mean ± SD) | Infat Oil (BPA) Diet (IO) (n = 8) (Mean ± SD) | T-Test (p Value) |
|---|---|---|---|
| Full volumetric bone mineral density (vBMD) (mg HA/cm3g) | 296.7 ± 14.85 | 306.58 ± 15.2 | 0.262 |
| (A) Cortical bone parameters | |||
| Tt.Ar (mm2) | 3.15 ± 0.07 | 3.07 ± 0.11 | 0.51 |
| Ct.Ar (mm2) | 1.75 ± 0.1 | 1.83 ± 0.12 | 0.31 |
| Ct.Ar/Tt.Ar (%) | 55 ± 0.02 | 59 ± 0.04 | 0.058 |
| Ct.Th (mm) | 0.31 ± 0.01 | 0.35 ± 0.03 | 0.026 |
| (B) Trabecular bone parameters (tROI) | |||
| BV/TV (%) | 3.9 ± 1.31 | 5.26 ± 2.02 | 0.17 |
| Tb.Th (mm) | 0.05 ± 0.004 | 0.06 ± 0.01 | 0.11 |
| Tb.N (mm-1) | 1.06 ± 0.15 | 1.19 ± 0.26 | 0.28 |
| Tb.Sp (mm) | 0.96 ± 0.14 | 0.89 ± 0.15 | 0.41 |
| (B1) Proximal ROI (proximal metaphysis) | |||
| BV/TV (%) | 5.7 ± 2 | 7.5 ± 3 | 0.22 |
| Tb.Th (mm) | 0.05 ± 0.004 | 0.06 ± 0.01 | 0.11 |
| Tb.N (mm−1) | 1.46 a ± 0.2 | 1.54 ± 0.29 | 0.55 |
| Tb.Sp (mm) | 0.71 ± 0.09 | 0.69 ± 0.11 | 0.73 |
| (B2) Distal ROI (proximal metaphysis) | |||
| BV/TV (%) | 0.72 ± 0.15 | 1.25 ± 0.5 | 0.015 |
| Tb.Th (mm) | 0.05 ± 0.0 | 0.05 ± 0.01 | 0.98 |
| Tb.N (mm−1) | 0.71 ± 0.1 | 0.84 ± 0.21 | 0.154 |
| Tb.Sp (mm) | 1.42 ± 0.21 | 1.25 ± 0.23 | 0.21 |
| (C) Bone biomechanical properties | |||
| Stiffness (N/mm) | 41.68 ± 14.4 | 36.37 ± 7.6 | 0.43 |
| Maximal load (N) | 22.8 ± 4.3 | 21.54 ± 3.5 | 0.53 |
| Energy to maximum (N*mm) | 14.02 ± 3.5 | 16.5 ± 5.0 | 0.3 |
Superscripts denote significant between-group differences as follows (p < 0.05); cortical thickness and the bone volume fraction (BV/TV) of the distal bone were significantly different. vBMD, full volumetric bone mineral density; Tt.Ar, total area; Ct.Ar, cortical area; Ct.Th, cortical thickness; BV/TV, bone volume fraction; Tb.Th, trabecular thickness; Tb.N, trabecular number, Tb.Sp, trabecular separation.