Table 1.
Composition of the experimental diets
| Diets, g/kg | ||||||
|---|---|---|---|---|---|---|
| Ingredients | Diet 1 | Diet 2 | Diet 3 | Diet 4 | Diet 5 | Diet 6 |
| Anchovy fish meala | 68.00 | 68.00 | 68.00 | 68.00 | 68.00 | 68.00 |
| Caseina | 30.00 | 30.00 | 30.00 | 30.00 | 30.00 | 30.00 |
| Gelatina | 39.90 | 39.90 | 39.90 | 39.90 | 39.90 | 39.90 |
| Amino acid mixb | 150.22 | 150.22 | 150.22 | 150.22 | 150.22 | 150.22 |
| α-Starch | 280.00 | 280.00 | 280.00 | 280.00 | 280.00 | 280.00 |
| Anchovy fish oilc | 22.80 | 22.80 | 22.80 | 22.80 | 22.80 | 22.80 |
| Soybean oil | 18.90 | 18.90 | 18.90 | 18.90 | 18.90 | 18.90 |
| Vitamin premixd | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 |
| Mineral premixe | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Ca(H2PO4)2 | 22.90 | 22.90 | 22.90 | 22.90 | 22.90 | 22.90 |
| Choline chloride (500 g/kg) | 6.00 | 6.00 | 6.00 | 6.00 | 6.00 | 6.00 |
| α-Cellulose | 150.00 | 150.00 | 150.00 | 150.00 | 150.00 | 150.00 |
| Ethoxyquin (300 g/kg) | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 | 0.50 |
| DL-Methionine | 0.00 | 2.02 | 4.04 | 6.06 | 8.08 | 10.10 |
| Glycine | 49.09 | 48.08 | 47.07 | 46.06 | 45.05 | 44.04 |
| Corn starch | 131.69 | 130.68 | 129.67 | 128.66 | 127.65 | 126.64 |
aFish meal was purchased from Pesquera Lota Protein Ltd. (Lota, Chile), casein was purchased from Hulunbeier Sanyuan Milk Co., Ltd. (Inner Mongolia, China), gelatin was purchased from Rousselot Gelatin Co., Ltd. (Guangdong, China)
bAmino acid mix (g/kg): arginine (100%), 11.80 g; histidine (73.3%), 7.23 g; isoleucine (99%), 11.82 g; leucine (99%), 18.99 g; lysine (78.8%), 15.99 g; cystine (99%), 0.81 g; phenylalanine (99%), 12.53 g; tyrosine (99%), 10.00 g; threonine (98%), 11.22 g; tryptophan (98%), 3.27 g; valine (99%), 14.24 g; glutamic acid (99%), 32.32 g
cFish oil was purchased form CIA. Pesquera Camanchaca S.A. (Santiago, Chile)
dPer kilogram of vitamin premix (g/kg): retinyl acetate (500,000 IU/g), 0.800 g; cholecalciferol (500,000 IU/g), 0.480 g; DL-α-tocopherol acetate (500 g/kg), 20.000 g; menadione (23%), 0.220 g; thiamine hydrochloride (98%), 0.120 g; riboflavin (80%), 0.990 g; pyridoxine hydrochloride (98%), 0.620 g; cyanocobalamin (1%), 0.100 g; niacin (99%), 2.58 g; D-biotin (2%), 5.000 g; meso-inositol (99%), 52.330 g; folic acid (96%), 0.520 g; ascorhyl acetate (93%), 7.160 g; calcium-D-pantothenate (90%), 2.780 g. All ingredients were diluted with corn starch to 1 kg
ePer kilogram of mineral premix (g/kg): FeSO4·H2O, 25.00 g; CuSO4·5H2O, 0.60 g; ZnSO4·H2O, 4.35 g; MnSO4·H2O, 2.04 g; KI, 1.10 g; NaSeO3, 2.50 g; MgSO4·H2O, 230.67 g. All ingredients were diluted with corn starch to 1 kg