Table 6.
Effects of methionine on malondialdehyde (MDA), protein carbonyl (PC) content, anti-superoxide anion (ASA) and anti-hydroxy radical (AHR) activities in the hepatopancreas and intestine of sub-adult grass carp1
| Met, g/kg diet | 2.21 | 4.24 | 6.22 | 8.25 | 10.24 | 12.26 |
|---|---|---|---|---|---|---|
| Hepatopancreas | ||||||
| MDA, nmol/mg protein | 2.19 ± 0.18b | 2.09 ± 0.10b | 1.15 ± 0.11a | 2.04 ± 0.14b | 2.15 ± 0.15b | 2.08 ± 0.08b |
| PC, nmol/mg protein | 6.63 ± 0.48c | 3.66 ± 0.31b | 2.56 ± 0.20a | 3.53 ± 0.36b | 3.67 ± 0.29b | 3.63 ± 0.14b |
| ASA, U/g protein | 197.0 ± 15.3 | 194.4 ± 8.7 | 202.9 ± 12.9 | 205.6 ± 15.2 | 202.9 ± 16.80 | 202.5 ± 7.3 |
| AHR, U/mg protein | 208.5 ± 8.0a | 229.5 ± 9.7c | 259.7 ± 6.4e | 256.0 ± 10.6e | 244.7 ± 5.90d | 219.3 ± 10.1b |
| Intestine | ||||||
| MDA, nmol/mg protein | 1.73 ± 0.14b | 1.72 ± 0.17b | 1.44 ± 0.08a | 1.41 ± 0.11a | 1.98 ± 0.12c | 2.09 ± 0.12c |
| PC, nmol/mg protein | 4.27 ± 0.34b | 3.63 ± 0.36a | 3.16 ± 0.38a | 3.19 ± 0.39a | 3.19 ± 0.26a | 5.30 ± 0.37c |
| ASA, U/g protein | 213.5 ± 19.0a | 281.6 ± 19.4b | 303.0 ± 24.5b | 284.9 ± 20.7b | 282.1 ± 25.2b | 287.3 ± 23.5b |
| AHR, U/mg protein | 133.4 ± 8.7a | 173.2 ± 13.0b | 198.1 ± 12.8c | 179.6 ± 11.9b | 175.8 ± 12.8b | 143.2 ± 12.7a |
| Regressions | ||||||
| Y PC in Hepatopancreas = 0.087X2–1.455X + 8.910 | R 2 = 0.773 | P = 0.108 | ||||
| Y AHR in Hepatopancreas = −1.757X2 + 26.793X + 155.081 | R 2 = 0.952 | P = 0.010 | ||||
| Y MDA in Intestine = 0.018X2–0.219X + 2.181 | R 2 = 0.745 | P = 0.129 | ||||
| Y PC in Intestine = 0.069X2–0.945X + 6.198 | R 2 = 0.855 | P = 0.055 | ||||
| Y ASA in Intestine = −1.819X2 + 31.349X + 165.218 | R 2 = 0.773 | P = 0.108 | ||||
| Y AHR in Intestine = −2.108X2 + 31.047X + 77.708 | R 2 = 0.929 | P = 0.019 | ||||
a-eMeans in the same row with different superscript letters differ significantly (P < 0.05)
1Values are mean ± SD (n = 6)