Figure 3.

Indirect immunofluorescence localization of Pma1-10. Cells were shifted to 37°C for 3 h, and then fixed, permeabilized, and stained with polyclonal anti-Pma1 and monoclonal anti-V-ATPase antibodies. (A) Pma1 localization in wild-type (L3852), pma1-10 end4–1 (XGX42–8B), pma1-10 (XGX28–1C), and pma1-10 pep4Δ (XGX28–1A) cells. Colocalization of Pma1 and V-ATPase at the vacuolar membrane is seen in pma1-10 pep4 cells. (B) Double labeling with anti-Pma1 and anti-V-ATPase in pma1-10 vps27Δ (XGX47–1D). Pma1 colocalizes in a large punctate spot with V-ATPase.