Figure 5.

At 37°C, Pma1-10 does not associate with a Triton-insoluble fraction. Wild-type (XGX28–1D) and pma1-10 pep4Δ (XGX28–1A) cells were pulse-labeled for 5 min at 37°C with Expre³⁵S³⁵S and chased for various times. Lysates were prepared, extracted with ice-cold Triton X-100, and separated into Triton-soluble and Triton-insoluble fractions, as described in Materials and Methods. T, total lysate; S, Triton-soluble; P, Triton-insoluble. Pma1 was immunoprecipitated and analyzed by SDS/PAGE and fluorography.