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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Methods Mol Biol. 2017;1596:27–41. doi: 10.1007/978-1-4939-6940-1_2

Fig. 2.

Fig. 2

Examples of viable circular permutants for AFF and fragments for FREX. (a) Stable and functional CPs were generated by cleaving the RBP sequences at the eight surface loops centered around the positions indicated by black spheres, and joining the original termini by a Gly/Ala/Ser-based linker of 30 amino acids. The ribose ligand is shown as black sticks. (b) The Fn3-FREX sensor was created by duplicating residues 48 to the C-terminus (black segment). The binding mutation site (Tyr87) contacts the target ligand (SH2 domain, sticks) and the tuning mutation site (IIe75) packs against hydrophobic residues in the two gray beta strands shown