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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Methods Mol Biol. 2017;1596:27–41. doi: 10.1007/978-1-4939-6940-1_2

Fig. 3.

Fig. 3

Simulated chemical (left) and thermal (right) denaturation curves illustrating how Cm and Tm values are calculated and interpolated, respectively. Lines are best fits of the data to the linear extrapolation equation (left) and to linear portions of the native and unfolded baselines (right)