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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Methods Mol Biol. 2017;1596:27–41. doi: 10.1007/978-1-4939-6940-1_2

Fig. 4.

Fig. 4

Cloning strategy for constructing AFF genes, (a) Annealing sites and compositions of primers 1–6 are indicated below the POI gene. The restriction enzyme digestion/ligation and fusion PCR methods for connecting the N and N′ half-genes are shown in (b, c), respectively