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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Kidney Int. 2010 Jul 21;78(9):868–882. doi: 10.1038/ki.2010.212

Figure 6. Chloride intracellular channel protein 5 (CLIC5) expression in podocytes is absent in CLIC5−/− mice.

Figure 6

(a) Whole cellular lysates (WCLs) were produced from isolated glomeruli, and CLIC5A and CLIC5B expression levels were determined by western blot analysis. Actin immunoblotting served as a loading control to enable protein expression comparisons between samples. Expression of CLIC5A and CLIC5B decreased between CLIC5+/+, CLIC5+/−, and CLIC5−/− mice. There was no detectable expression of either CLIC5 isoform in CLIC5−/− mice. (b) Mouse kidney sections were double immunofluorescently labeled with CLIC5 (A and B) and WT1 (C and D) antibodies, and nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, merge, E and F). CLIC5+/+ glomeruli exhibited CLIC5 expression, whereas no CLIC5 was evident in kidney sections from CLIC5−/− mice. Immunofluorescence for CLIC5, WT1, and DAPI was merged, indicating that CLIC5 expression was absent in podocytes of CLIC5−/− mice. Images were taken at × 60 magnification.