Table 1.
List of proteins identified in the slit diaphragm-enriched fraction
| Fraction | Approximate MW range |
Entry | Description | No. of peptides |
Percentage coverage |
Mascot score |
|---|---|---|---|---|---|---|
| P1 | 300–250 | PLEC1_mouse | Plectin-1 | 16 | 5 | 270 |
| FINC_mouse | Fibronectin | 2 | 1 | 61 | ||
| FLNA_mouse | Filamin-A | 15 | 8 | 391 | ||
| FLNB_mouse | Filamin-B | 10 | 4 | 149 | ||
| P2 | 250–200 | NPHN_rat | Nephrin | 5 | 6 | 187 |
| SPTA2_rat | Spectrin-α chain, brain | 70 | 61 | 3005 | ||
| SPTB2_mouse | Spectrin-β chain, brain 1 | 61 | 52 | 2270 | ||
| MYH9_rat | Myosin-9 | 59 | 52 | 2277 | ||
| MYH10_mouse | Myosin-10 | 19 | 15 | 551 | ||
| TLN1_mouse | Talin-1 | 12 | 5 | 191 | ||
| TLN2_mouse | Talin-2 | 5 | 5 | 49 | ||
| ZO1_mouse | ZO-1 | 2 | 1 | 62 | ||
| P3 | 200–170 | MYH11_mouse | Myosin-11 | 18 | 10 | 457 |
| MYO6_mouse | Myosin-VI | 8 | 8 | 148 | ||
| CLH_mouse | Clathrin heavy chain | 4 | 4 | 60 | ||
| P4 | 170–120 | AT1A1_mouse | Sodium/potassium-transporting ATPase subunit α-1 | 12 | 22 | 389 |
| SYNPO_rat | Synaptopodin | 12 | 24 | 259 | ||
| KIRR1_rat | Kin of IRRE-like protein 1 | 2 | 5 | 65 | ||
| ARVC_mouse | Armadillo repeat protein deleted in velo-cardio-facial syndrome homolog |
1 | 1 | 56 | ||
| MYO1B_rat | Myosin-lb | 2 | 3 | 39 | ||
| MYO1C_mouse | Myosin-lc | 31 | 48 | 946 | ||
| VINC_mouse | Vinculin | 13 | 17 | 190 | ||
| CTND1_mouse | Catenin δ-1 | 10 | 12 | 186 | ||
| MYO1D_rat | Myosin-ld | 13 | 19 | 173 | ||
| MYO1E_rat | Myosin-le | 3 | 3 | 101 | ||
| NCKP1_mouse | Nck-associated protein 1 | 4 | 3 | 86 | ||
| P5 | 120–90 | ACTN4_mouse | α-Actinin-4 | 24 | 66 | 1533 |
| ACTN1_rat | α-Actinin-1 | 21 | 52 | 1265 | ||
| CTNA1_mouse | Catenin α-1 | 19 | 52 | 784 | ||
| CTNB1 | Catenin β-1 | 11 | 23 | 272 | ||
| AP2A2_rat | AP-2 complex subunit α-2 | 18 | 37 | 380 | ||
| AP2B1_mouse | AP-2 complex subunit β-1 | 7 | 17 | 111 | ||
| CTNA3_mouse | Catenin α-3 | 2 | 4 | 56 | ||
| GIT1_mouse | ARF GTPase-activating protein GIT-1 | 3 | 6 | 45 | ||
| P6 | 90–65 | GRP78_mouse | 78 kDa glucose-regulated protein precursor | 7 | 18 | 254 |
| MOES_mouse | Moesin | 15 | 42 | 418 | ||
| GELS_rat | Gelsolin | 6 | 12 | 72 | ||
| EZRLrat | Ezrin | 15 | 45 | 408 | ||
| RADI_mouse | Radixin | 12 | 32 | 335 | ||
| HS12B_mouse | Heat shock 70 kDa protein 12B | 9 | 23 | 168 | ||
| HS12A_mouse | Heat shock 70 kDa protein 12A | 3 | 5 | 59 | ||
| HSP7C_CRIGR | Heat shock cognate 71 kDa protein | 12 | 29 | 351 | ||
| DTNB_rat | Dystrobrevin-β | 9 | 17 | 223 | ||
| TCPA_CRIGR | T-complex protein-1 subunit-α | 1 | 5 | 53 | ||
| EFR3A_mouse | Protein EFR3 homolog A | 2 | 4 | 59 | ||
| PLAK_mouse | Junction plakoglobin | 10 | 23 | 205 | ||
| CALD1_rat | Non-muscle caldesmon | 3 | 6 | 38 | ||
| FXR1_mouse | Fragile X mental retardation syndrome-related protein 1 | 1 | 2 | 42 | ||
| P7 | 65–55 | VIME_rat | Vimentin | 10 | 45 | 511 |
| PLVAP_rat | Plasmalemma vesicle-associated protein | 12 | 46 | 438 | ||
| SNTB2_mouse | β2-syntrophin | 14 | 44 | 627 | ||
| SNTA1_mouse | α1-syntrophin | 4 | 12 | 53 | ||
| TCPD_mouse | T-complex protein 1 subunit-δ | 4 | 9 | 134 | ||
| 2AAA_mouse | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A-α isoform |
5 | 14 | 123 | ||
| TCPQ_mouse | T-complex protein 1 subunit-θ | 4 | 8 | 113 | ||
| TCPH_mouse | T-complex protein 1 subunit-φ | 4 | 11 | 100 | ||
| FYN_mouse | Tyrosine kinase Fyn | 3 | 7 | 97 | ||
| SRC_mouse | Tyrosine kinse Src | 3 | 7 | 96 | ||
| YES_mouse | Tyrosine kinase Yes | 7 | 20 | 83 | ||
| COR2B_mouse | Coronin-2B | 5 | 10 | 87 | ||
| TCPZ_mouse | T-complex protein 1 subunit ζ | 3 | 11 | 87 | ||
| TCPE_mouse | T-complex protein 1 subunit-ε | 1 | 3 | 66 | ||
| EHD1_mouse | EH domain-containing protein-1 | 2 | 5 | 61 | ||
| VATB2_mouse | Vacuolar ATP synthase subunit B, brain isoform | 10 | 30 | 363 | ||
| AP2M1 | AP-2 complex subunit μ-1 | 8 | 21 | 178 | ||
| DESM_rat | Desmin | 6 | 21 | 175 | ||
| TBB5_CRIGR | Tubulin β-5 chain | 5 | 30 | 117 | ||
| TBA1 B_CRIGR | Tubilin α-1B chain | 6 | 17 | 90 | ||
| SAM50_mouse | Sorting and assembly machinery component 50 homolog | 3 | 8 | 59 | ||
| TCPB_mouse | T-complex protein 1 subunit-β | 3 | 8 | 42 | ||
| P8 | 55–45 | PODO_rat | Podocin | 10 | 54 | 298 |
| SERPH | Serpin H1 | 5 | 23 | 242 | ||
| FLOT1_mouse | Flotillin-1 | 8 | 30 | 241 | ||
| FLOT2_mouse | Flotillin-2 | 7 | 21 | 146 | ||
| ARP3_mouse | Actin-related protein 3 | 6 | 41 | 142 | ||
| EF1G_rat | Elongation factor 1-γ | 3 | 10 | 76 | ||
| SNX8_mouse | Sorting nexin-8 | 1 | 2 | 73 | ||
| GNAS1_rat | Guanine nucleotide-binding protein Gs subunit-α isoform XLas | 3 | 4 | 73 | ||
| HA1 B_mouse | H-2 class I histocompatibility antigen, K-α chain | 3 | 8 | 64 | ||
| NHRF2_rat | Na+/H+ exchange regulatory cofactor NHE-RF2 | 3 | 14 | 49 | ||
| ILK_mouse | Integrin-linked protein kinase | 3 | 8 | 107 | ||
| P9 | 45–37 | ACTG_mouse | Actin, cytoplasmic 2 | 10 | 81 | 1743 |
| TMOD3_mouse | Tropomodulin-3 | 6 | 22 | 94 | ||
| PARVA_mouse | α-parvin | 3 | 10 | 149 | ||
| PARVB_mouse | β-parvin | 1 | 3 | 79 | ||
| PP1B_mouse | Serine/threonine protein phosphatase PP1-β catalytic subunit | 6 | 34 | 263 | ||
| LIMS2_mouse | LIM and senescent cell antigen-like-containing domain protein 2 | 5 | 16 | 83 | ||
| PP1A_rat | Serine/threonine protein phosphatase PP1-α catalytic subunit | 7 | 41 | 261 | ||
| PP1G_mouse | Serine/threonine protein phosphatase PP1-γ catalytic subunit | 6 | 45 | 231 | ||
| ANXA2_rat | Annexin A2 | 7 | 36 | 178 | ||
| CAZA2_mouse | F-actin-capping protein subunit α-2 | 5 | 26 | 175 | ||
| CAZA1_mouse | F-actin-capping protein subunit α-1 | 6 | 41 | 120 | ||
| GNAI2_rat | Guanine nucleotide-binding protein Gi, α-2 subunit | 4 | 21 | 80 | ||
| AAKG1_mouse | 5′-AMP-activated protein kinase subunit γ-1 | 1 | 5 | 59 | ||
| P10 | 37–32 | GBB2_mouse | Guanine nucleotide-binding protein Gi/Gs/Gt subunit β-2 | 9 | 62 | 391 |
| GBB1_mouse | Guanine nucleotide-binding protein Gi/Gs/Gt subunit β-1 | 6 | 40 | 349 | ||
| AQP1_mouse | Aquaporin-1 | 3 | 13 | 84 | ||
| KC1A_mouse | Casein kinase I isoform-α | 2 | 5 | 55 | ||
| PP2AA_mouse | Serine/threonine-protein phosphatase 2A catalytic subunit-α | 1 | 3 | 54 | ||
| LPP1_rat | Lipid phosphate phosphohydrolase 1 | 1 | 7 | 53 | ||
| LPP3_rat | Lipid phosphate phosphohydrolase 3 | 2 | 9 | 38 | ||
| STX4_mouse | Syntaxin-4 | 3 | 13 | 48 | ||
| ARPC2 | Actin-related protein 2/3 complex subunit 2 | 7 | 24 | 108 | ||
| C1O9A_mouse | Coiled-coil domain-containing protein 109A | 2 | 12 | 90 | ||
| TPM4_rat | Tropomyosin α-4 | 3 | 14 | 75 | ||
| K0152_mouse | Uncharacterized protein KIAA0152 | 2 | 8 | 80 | ||
| P11 | 32–28 | CLIC5_mouse | Chloride intracellular channel protein 5 | 7 | 50 | 216 |
| VAPB_mouse | Vesicle-associated membrane protein-associated protein B | 2 | 13 | 123 | ||
| VAPA_mouse | Vesicle-associated membrane protein-associated protein A | 3 | 23 | 111 | ||
| RSU1_mouse | Ras suppressor protein 1 | 6 | 46 | 86 | ||
| RAB5C_mouse | Ras-related protein Rab-5c | 2 | 10 | 57 | ||
| TM111_mouse | Transmembrane protein 111 | 3 | 14 | 94 | ||
| P12 | 28–23 | RAB5B_mouse | Ras-related protein Rab-5B | 3 | 17 | 81 |
| RAB5C_mouse | Ras-related protein Rab-5C | 5 | 42 | 238 | ||
| RAB5A_mouse | Ras-related protein Rab-5A | 5 | 33 | 161 | ||
| HSPB1 | Heat shock protein β-1 | 5 | 38 | 131 | ||
| RAB21_mouse | Ras-related protein Rab-21 | 3 | 29 | 106 | ||
| SNP23_mouse | Synaptosomal-associated protein 23 | 3 | 25 | 91 | ||
| RRAS_mouse | Ras-related protein R-ras | 3 | 17 | 133 | ||
| RRAS2_mouse | Ras-related protein R-ras2 | 5 | 27 | 106 | ||
| MLRA_rat | Myosin regulatory light chain 2-A | 3 | 23 | 120 | ||
| THY1_rat | Thy-1 membrane glycoprotein | 3 | 17 | 68 | ||
| RAP1A_mouse | Ras-related protein Rap-1A | 1 | 6 | 54 | ||
| CAV1_mouse | Caveolin-1 | 4 | 24 | 102 | ||
| RAB18_mouse | Ras-related protein Rab-18 | 3 | 22 | 97 | ||
| P13 | 23–18 | RAP1B_rat | Ras-related protein Rap-1b | 4 | 36 | 215 |
| CDC42_rat | Cell division control protein 42 homolog | 5 | 36 | 171 | ||
| RAC1_mouse | Ras-related C3 botulinum toxin substrate 1 | 6 | 52 | 123 | ||
| RASH_mouse | GTPase HRas precursor | 3 | 28 | 99 | ||
| RASN_CAVPO | GTPase NRas | 3 | 26 | 76 | ||
| RHOA_mouse | Transforming protein ρA | 4 | 48 | 71 | ||
| TP4A1_mouse | Protein tyrosine phosphatase type IVa | 2 | 12 | 48 | ||
| RASK_mouse | GTPase KRas | 4 | 31 | 48 | ||
| MLRN_mouse | Myosin regulatory light chain 2 | 3 | 27 | 44 | ||
| MLRA_rat | Myosin regulatory light chain 2-A | 3 | 62 | 337 | ||
| RAP2B_mouse | Ras-related protein Rap-2b | 1 | 6 | 88 | ||
| MLRB_mouse | Myosin regulatory light chain 2-B | 3 | 62 | 367 | ||
| ARPC3_mouse | Actin-related protein 2/3 complex subunit 3 | 4 | 30 | 165 | ||
| ARPC4_mouse | Actin-related protein 2/3 complex subunit 4 | 4 | 46 | 94 | ||
| COF1_mouse | Cofilin-1 | 2 | 30 | 152 | ||
| P14 | 18–14 | MYL6_mouse | Myosin light chain polypeptide 6 | 3 | 66 | 422 |
| PDZ11_mouse | PDZ domain-containing protein 11 | 3 | 35 | 99 | ||
| AP2S1_mouse | AP-2 complex subunit σ-1 | 2 | 10 | 47 | ||
| P15 | 14–8 | DYL1_mouse | Dynein light chain 1 | 2 | 49 | 109 |
| UBIQ_CAVPO | Ubiquitin | 2 | 32 | 50 |
A mascot score of 32 or greater was considered significant.
The bold text highlights CLIC5 proteins.