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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2016 Jul 22;99:20–31. doi: 10.1016/j.freeradbiomed.2016.07.022

Fig 5. Meclizine prevents depletion of reduced glutathione (GSH) stores and attenuates ROS formation in hypoxic DRG neurons.

Fig 5

(A) GSH levels were assessed by fluorescent dye-mediated detection of thiol groups. GSH increases by meclizine in hypoxia and normoxia were abrogated by 2DG (diagonal lines). Values are given as mean±SEM of 4 independent experiments; P< 0.05; *indicates different from normoxia; ** different from meclizine in normoxia; †indicates different from normoxia; ††different from hypoxia; ‡different from hypoxia+meclizine. (B) Hypoxia induced decreases in GSH/GSSG ratios in DRG neurons are partially reversed by meclizine supplementation. Data are mean±SEM of 3 experiments; P< 0.05; *indicates different from normoxia; ** different from hypoxia. (C) ROS levels in normoxic and hypoxic DRG neurons with/out meclizine were compared by imaging fluorescence of superoxide-mediated oxidation of dihydroethidium to 2-hydroxyethidium(red). Fluorescence intensity normalized to cell surface area quantified by ImageJ is presented as mean±SEM intensity for 3 sets of experiments; in each experiment, 5–8 DRG neurons were scored for each condition (total 20 ≥ for each condition; scale bar=10 μm). Meclizine significantly attenuated hypoxia-induced fluorescence; *indicates different from normoxia, ** different from hypoxia without meclizine supplementation; P<0.05.