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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2016 Jul 22;99:20–31. doi: 10.1016/j.freeradbiomed.2016.07.022

Fig 8. Meclizine attenuates hypoxia-induced mitochondrial clustering and compromise of DRG neurites morphology.

Fig 8

Mitochondrial distribution is observed by immunofluorescence (IF) of cytochrome c oxidase subunit 1 (COX-1, green) while cell bodies and neurites are visualized by IF of neurofilament 200 (NF-200, red). Intense COX-1 IF in cell bodies reflects high mitochondrial content; overexposing COX-1 enabled visualization of the less dense mitochondria in neurites. Mostly uniform distribution was observed in neurites in normoxia [left]. Following hypoxia, COX-1 IF showed mitochondrial clustering. In cultures supplemented with meclizine [right], clustering and neurite fragmentation were reduced. Merged images of segments of neurites are shown at higher magnification (bottom panel) scale bar=10 μm.