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. 2017 Jul 25;7(7):e1179. doi: 10.1038/tp.2017.144

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Physiologically active neurons differentiated from human induced pluripotent stem cell (hiPSC)-derived NPCs. (a) Expression of presynaptic protein Synapsin1 in MAP2-positive neuronal cells. Scale bar=10 μm. (b) Representative graphs of electrophysiological recordings of Na⁺ and K⁺ currents, Bicuculline-sensitive GABA-mediated response and evoked action potentials in 8-week-old hiPSC-derived CTL1 neurons. (c) Spontaneous calcium oscillations are more apparent in 8-week-old neurons than in 3-week-old neuronal cultures. Left, representative Ca²⁺-transients of individual neurons; right, quantification of Ca²⁺-transients 3 and 8 weeks after terminal differentiation. t-test, *P<0.0001. (d) Presence of synaptic cleft (arrow) and presynaptic vesicles (arrowhead) was verified by electron microscope imaging in 5-week-old hiPSC-derived neurons. Scale bar=500 nm.