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. 2017 Jul 25;7(7):e1179. doi: 10.1038/tp.2017.144

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Reduced EHMT1 mRNA expression and altered gene expression in human induced pluripotent stem cell (hiPSC)-derived neuronal cells from KS-ASD patient on day 10 of terminal differentiation. (a) The mRNA level of EHMT1 transcript variant 1 is reduced in KS-ASD cells compared to CTL1 and CTL2 cells, whereas no change in the level of EHMT1 transcript variant 2 is observed in KS-ASD vs control cells. t-test, CTL1 vs KS-ASD *P=0.0061; CTL2 vs KS-ASD ^#P=0.0111. Right panel, Western blot analysis of EHMT1 protein expression in CTL1, CTL2 and KS-ASD neurons. GAPDH was used as protein-loading control. (b) Gene expression changes in KS-ASD neurons compared to CTL1 (left) or CTL2 (right) neuronal cells on day 10 of terminal differentiation. Mann–Whitney test, *P<0.05, **P<0.005.