Figure 3.

Effect of ethyl acetate fraction of E. pavieana on inducible nitric oxide synthase expression in lipopolysaccharide-stimulated macrophages. (a) Cells were incubated with various concentrations of ethyl acetate fraction of E. pavieana in the presence of 1 μg/mL lipopolysaccharide for 24 h. Cells were lysed, and inducible nitric oxide synthase as well as β-actin protein levels were determined by Western blot analysis. The immunoblot picture shown is a representative of three separate experiments. (b) Cells were incubated with various concentrations in the presence or absence of 1 μg/mL lipopolysaccharide for 12 h. Total RNA was extracted, and inducible nitric oxide synthase as well as β-actin mRNA levels were determined by reverse transcription-polymerase chain reaction. The gel picture shown is a representative of three separate experiments. *P < 0.05 compared to the lipopolysaccharide-treated cells (a: 690 mm × 234 mm; b: 707 mm × 281 mm)