Figure 4.

Effect of ethyl acetate fraction of E. pavieana on nuclear factor-kappa B p65 subunit nuclear translocation in lipopolysaccharide-stimulated macrophages. Cells were incubated with various concentrations of ethyl acetate fraction of E. pavieana and 1 μg/mL Lipopolysaccharide for 2 h. Nuclear protein extracts were prepared and analyzed by Western blot analysis. The gel picture shown is a representative of three separated experiments. *P < 0.05 compared to the Lipopolysaccharide-treated cells (684 mm × 254 mm)