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. Author manuscript; available in PMC: 2018 May 18.
Published in final edited form as: Cell. 2017 May 18;169(5):930–944.e22. doi: 10.1016/j.cell.2017.05.004

Figure 1. Acute and reversible depletion of CTCF with the auxin-inducible degron system in mESCs.

Figure 1

(A) Deploying the AID system at Ctcf in mESCs.

(B) Western-blot showing reversible loss of CTCF in CTCF-AID cells

(C) Immunofluorescence staining

(D) Long-term survival (12 days) is only compromised in CTCF-AID cells treated with auxin after introduction of the Tir1 transgene

(E) Time-course flow-cytometry

(F) Brightfield images of mESC colonies after auxin treatment indicating cells tolerate a 2-day depletion with no adverse effects on viability. See Figure S1