Figure 4. Proper insulation of TADs is not required for higher-order segregation of A and B compartments.
(A) Hi-C contact maps at 100kb resolution across entire chromosome 2. Bar denotes segments called as A (green) or B (red) compartment using 20kb-cis Eigenvector 1. Normalized Hi-C counts are multiplied by 105
(B) Distributions of cis Eigenvector 1 values across entire chromosome 2 are remarkably stable to depletion of CTCF
(C) cis Eigenvector 1 values are not affected genome-wide by CTCF depletion
(D) Overall scaling of Hi-C contact frequency as a function of genomic distance is not affect by the loss of CTCF, highlighting that CTCF does not affect general chromatin compaction. See Figure S4