Table 1.
qPCR for GlyR subunits in the nucleus accumbens (nAc), hippocampus (HC), brainstem (BS) and spinal cord (SC)
| GlyR α1 | GlyR α2 | GlyR α3 | GlyR β | |
|---|---|---|---|---|
| Ct value | ||||
| nAc | 7.7 ± 0.4 | 11.1 ± 0.5 | 18.3 ± 0.7 | 11.1 ± 0.9 |
| HC | 14.0 ± 0.7 | 13.4 ± 0.6 | 20.5 ± 0.5 | 11.3 ± 1.0 |
| BS | 2.6 ± 0.5 | 11.9 ± 0.6 | 17.3 ± 0.4 | 9.6 ± 0.9 |
| SC | 3.4 ± 0.4 | 12.9 ± 0.2 | 16.6 ± 0.5 | 10.9 ± 0.9 |
| P‐value | GlyR α1 | GlyR α2 | GlyR α3 | GlyR β |
| nAc vs. HC | n.s. | n.s. | ||
| *0.025 | *0.045 | 0.18 | 2.27 | |
| nAc vs. BS | *0.026 | n.s. | n.s. | n.s. |
| 0.026 | 1.65 | 0.91 | 0.20 | |
| nAc vs. SC | n.s. | n.s. | n.s. | |
| *0.017 | 0.09 | 0.24 | 6.00 | |
| HC vs. BS | n.s. | |||
| HC vs. BS | ***0.0002 | *0.018 | **0.002 | 0.050 |
| HC vs. SC | n.s. | n.s. | ||
| **0.002 | 1.15 | ***0.0003 | 1.16 | |
| BS vs. SC | n.s. | n.s. | n.s. | n.s. |
| 1.34 | 0.70 | 0.31 | 0.17 | |
| P‐value | nAc | HC | BS | SC |
| α1 vs. α2 | *0.021 |
n.s. 1.83 |
*** 0.0003 | **0.0023 |
| α1 vs. α3 | **0.002 | ***0.0006 | ***0.00002 | ***0.0003 |
| α1 vs. β | **0.002 | **0.0017 | **0.0025 | *0.018 |
| α2 vs. α3 |
n.s. 0.17 |
*0.031 | **0.0024 | *0.010 |
| α2 vs. β |
n.s. 4.81 |
n.s. 0.29 |
n.s. 0.08 |
n.s. 0.62 |
| α3 vs. β | *0.031 | **0.0030 | **0.0035 | **0.0066 |
Quantitative real‐time PCR of GlyR in HC, BS and SC of DRD1/GFP mice. Threshold cycles were normalized to GAPDH and primer efficiency was accounted for (values are mean ± SEM; n = 4; Kruskal–Wallis and unpaired post hoc Bonferroni t test: *** P < 0.01, ** P < 0.01, * P < 0.05, n.s. not significant).