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. 2017 Jul 5;595(15):5265–5284. doi: 10.1113/JP274275

Figure 8. EGTA and inhibitors of nSMase impair ATP‐independent endocytosis at near physiological temperature.

Figure 8

A, sampled Cm responses (Aa) to depol1msX500 from calyces dialysed with 10 mm EGTA, or ATPγS and EGTA, at 34–35°C. Ab, average Cm traces after normalization to ΔCm are from control (n = 12), EGTA (n = 8), ATPγS (n = 8) and ATPγS and EGTA (n = 8). B, sampled Cm responses (Ba) to depol1msX500 under dialysis with ATPγS and DMSO (0.1%), ATPγS and 10 μm spiroepoxide, or ATPγS and 30 μm sphingolactone‐24 at 34–35°C. Bb, average Cm traces after normalization to ΔCm are from ATPγS and DMSO (n = 8), ATPγS and spiroepoxide (n = 9) and ATPγS and sphingolactone‐24 (n = 9).