Table 1.
Stimulation | Treatment | QCa (pC) | ΔCm (fF) | Rate_endo (fF s−1) | Endo25s (fF) |
---|---|---|---|---|---|
Depol50msX10 | Ctrl | 614 ± 33 | 1199 ± 80 | 430 ± 41 | 1939 ± 125 |
ATPγS | 637 ± 55 | 784 ± 99 | 141 ± 25 | 796 ± 69 | |
AMP‐PNP | 570 ± 44 | 735 ± 91 | 219 ± 63 | 935 ± 169 | |
EGTA | 884 ± 48 | 1348 ± 74 | 183 ± 27 | 1201 ± 87 | |
ATPγS + EGTA | 785 ± 36 | 599 ± 56 | 10 ± 3 | 59 ± 15 | |
T/C | 808 ± 87 | 631 ± 50 | 295 ± 60 | 1283 ± 272 | |
EGTA + T/C | 779 ± 49 | 839 ± 198 | 36 ± 10 | 417 ± 173 | |
ATPγS + T/C | 560 ± 68 | 805 ± 135 | 105 ± 37 | 609 ± 160 | |
ATPγS + Pitstop 1 | 627 ± 44 | 855 ± 78 | 149 ± 30 | 804 ± 146 | |
ATPγS + DNF | 687 ± 98 | 804 ± 112 | 132 ± 33 | 924 ± 297 | |
ATPγS + h‐COase | 612 ± 27 | 843 ± 73 | 144 ± 43 | 718 ± 56 | |
ATPγS + COase | 621 ± 29 | 869 ± 105 | 58 ± 17 | 334 ± 57 | |
EGTA + pitstop1 | 884 ± 77 | 1270 ± 186 | 83 ± 25 | 843 ± 155 | |
EGTA + DNF | 813 ± 53 | 883 ± 81 | 42 ± 4 | 507 ± 91 | |
T/C + Pitstop1 | 605 ± 39 | 431 ± 47 | 195 ± 45 | 968 ± 178 | |
DMSO | 630 ± 26 | 1107 ± 104 | 455 ± 38 | 1888 ± 153 | |
Spiro | 643 ± 26 | 1121 ± 89 | 285 ± 48 | 1347 ± 147 | |
Spingo | 647 ± 44 | 1118 ± 83 | 319 ± 46 | 1386 ± 128 | |
ATPγS + DMSO | 729 ± 67 | 1013 ± 110 | 149 ± 24 | 908 ± 135 | |
ATPγS + Spiro | 662 ± 40 | 1076 ± 96 | 63 ± 25 | 341 ± 55 | |
ATPγS + Sphingo | 734 ± 38 | 792 ± 111 | 69 ± 17 | 343 ± 86 | |
Depol20ms | Ctrl | 34 ± 3 | 443 ± 20 | 30 ± 3 | 378 ± 19 |
ATPγS | 36 ± 2 | 307 ± 33 | 3 ± 2 | 25 ± 12 | |
AMP‐PNP | 36 ± 2 | 320 ± 24 | 4 ± 1 | 20 ± 10 | |
0 mm GTP | 28 ± 4 | 385 ± 33 | 9 ± 4 | 69 ± 37 | |
3 mm GTP | 34 ± 2 | 371 ± 45 | 27 ± 5 | 324 ± 36 | |
ATPγS + 3 mm GTP | 34 ± 3 | 210 ± 48 | 2 ± 1 | 9 ± 8 | |
Pitstop 1 | 31 ± 1 | 424 ± 33 | 15 ± 3 | 176 ± 32 | |
DNF | 37 ± 1 | 412 ± 36 | 8 ± 2 | 198 ± 29 | |
DMSO | 38 ± 2 | 402 ± 33 | 29 ± 4 | 339 ± 35 | |
Spiro | 34 ± 2 | 378 ± 24 | 22 ± 3 | 278 ± 24 | |
Sphingo | 39 ± 3 | 423 ± 25 | 29 ± 3 | 363 ± 33 | |
SMasecyto | 33 ± 1 | 433 ± 11 | 38 ± 13 | 417 ± 22 | |
SMasebath | 34 ± 2 | 370 ± 57 | 34 ± 2 | 353 ± 37 |