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M. smegmatis biofilms formed in TSB media supplemented with 1% procalamine and incubated at 30 °C for 72 h with no disturbance. Wild-type strain compared with SHP1 (mscR−), SHP1C (complemented SHP1), S24 (mshC−), S24C (complemented S24), R119 (egtA−), and R119C (complemented R119).
