TABLE 2.
Strain | OD630 | P-value* |
---|---|---|
Wild type | 1.153 ± 0.012 | |
A1 (mshA−) | 1.057 ± 0.028 | 0.01514 |
A1C | 1.116 ± 0.020 | 0.0180** |
S24 (mshC)− | 0.852 ± 0.132 | 0.04119 |
S24C | 1.159 ± 0.034 | 0.88105 |
R119 (egtA)− | 1.188 ± 0.003 | 0.02736 |
R119C | 1.054 ± 0.030 | 0.01847 |
SHP1 (mscR)− | 0.713 ± 0.101 | 0.00249 |
SHP1C | 1.120 ± 0.009 | 0.03352 |
Biofilms were assayed at OD630 for four replicates.
P-value when compared with wild type calculated using a paired Student’s two-tailed t-test.
Partial complementation due to inducible nature of the acetamidase promoter controlling the mshA expression in pALACE vector (22).